After a mission trip to Arlington, Texas, Calvary Baptist Church members were inspired to establish a nonprofit providing charitable dental care to Lexington residents in need. Calvary partnered with Faith Lutheran Church and together Mission Lexington, Inc. was incorporated in March 2005.

In June 2006 the first patient was seen in the Mission Lexington Dental Clinic. The clinic was staffed by one part-time coordinator, and all provider positions were filled by volunteers from Calvary Baptist Church, Faith Lutheran Church, and various community members.

The Medical Clinic opened in December 2008 in partnership with Good Shepherd Episcopal Church. In 2013 construction of the Newtown Pike Extension demolished the building that housed Nathaniel Mission, a charitable healthcare clinic that had provided services to the community since 1979. All of Nathaniel Mission’s patients were taken in by Mission Lexington nearly doubling our the size of the patients seen annually.

In 2015 Faith Pharmacy relocated to Mission Lexington and became the third health clinic of the organization. The pharmacy was established in 2000 by Maxwell Street Presbyterian Church and Christ the King Cathedral and fills prescriptions free of charge for patients referred by physicians and health clinics. The pharmacy is staffed by community pharmacists, UK student pharmacists, faculty and alumni each Saturday morning.

In August of 2017 the Vision Clinic opened and saw its first patient.

In 2019 the Board of Directors approved to change the name of the organization to Mission Health Lexington to more effectively capture the array of clinics and services.

Mission Health Lexington provides comprehensive charitable healthcare to Fayette County adult residents living without insurance and below federal poverty guidelines.